The Tesco near Brewery Court in Cirencester is set to close.
The Farrell Close supermarket will shut in October and be replaced by a smaller Express store.
READ MORE: Shoppers react to Tesco Metro in Cirencester closing
A Tesco spokesperson said the new store, which is believed to be located on the site of the old Dorothy Perkins on Cricklade Street, would 'better serve the needs of customers' in the town centre.
However, a number of jobs are thought to be at risk.
A Tesco spokesperson said: “We have taken the difficult decision to close our Cirencester Superstore and have done so after concluding that a new Express format store would better serve the needs of customers in Cirencester town centre.
"We realise this will be unsettling for our colleagues and we will work hard to find alternative roles in our nearby stores.”
The new Express store will open at the same time as the Superstore closure.
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